Thyroid Hormone Tests

T3 (Triiodothyronine) Test, T4 (Thyroxine) Test, Thyroxine (T4) Test, Triiodothyronine (T3) Test

Test Overview

Thyroid hormone tests are blood tests that check how well the thyroid gland is working. The thyroid gland makes hormones that regulate the way the body uses energy.

The thyroid gland is a butterfly-shaped gland that lies in front of your windpipe (trachea), just below your voice box (larynx). The thyroid gland uses iodine from food to make two thyroid hormones, thyroxine (T4) and triiodothyronine (T3). The thyroid gland stores these thyroid hormones and releases them as they are needed.

Thyroid hormones are needed for normal development of the brain, especially during the first 3 years of life. Mental retardation may occur if a baby's thyroid gland does not produce enough thyroid hormone (congenital hypothyroidism). Older children also need thyroid hormones to grow and develop normally, and adults need the hormones to regulate the way the body uses energy (metabolism). The United States Preventive Services Task Force recommends that all newborns be tested for congenital hypothyroidism.¹

Thyroid hormone blood tests include:

• Total thyroxine (T4). Most of the thyroxine (T4) in the blood is attached to a protein called the thyroxine-binding globulin. Less than 1% of the T4 is unattached. A total T4 blood test measures both bound and free thyroxine. Unattached thyroxine affects tissue function in the body, but attached thyroxine does not.
• Free thyroxine (FTI or FT4). Free thyroxine (T4) can be measured directly (FT4) or calculated as the free thyroxine index (FTI). The FTI tells how much T4 is present compared to the thyroxine-binding globulin. The FTI can help tell if abnormal amounts of T4 are present because of abnormal amounts of thyroxin-binding globulin.
• Triiodothyronine (T3). Most of the T3 in the blood is attached to the thyroxine-binding globulin. Less than 1% of the T3 is unattached. A T3 blood test measures both bound and free triiodothyronine. T3 has a greater effect on the way the body uses energy than T4, even though T3 is normally present in smaller amounts than T4.

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Author:	Caroline Rea, RN, BS, MS	Last Updated: December 3, 2007
Medical Review:	Caroline S. Rhoads, MD - Internal Medicine David C.W. Lau, MD, PhD, FRCPC - Endocrinology & Metabolism
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